ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information. Citations from scientific literature and patents are provided for informational purposes only. While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy.
#Dim3 to d1b sspr license#
Any proposed commercial use is prohibited without a license from ATCC. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. This product is intended for laboratory research use only. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. The product is provided 'AS IS' and the viability of ATCC ® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information. If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 xg for 5 to 10 minutes. It is not necessary to remove the cryoprotective agent. A 5% CO 2 in air atmosphere is recommended if using the medium described on this product sheet. Incubate the culture at 37☌ in a suitable incubator.
It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Ĥ. It is important to avoid excessive alkalinity of the medium during recovery of the cells. Transfer the vial contents to a 75 cm 2 tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio). All of the operations from this point on should be carried out under strict aseptic conditions.ģ. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol.
Thawing should be rapid (approximately 2 minutes).Ģ. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thaw the vial by gentle agitation in a 37☌ water bath. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vessel exploding or blowing off its cap with dangerous force creating flying debris.ġ. It is important to note that some vials leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen.
#Dim3 to d1b sspr full#
SAFETY PRECAUTION : ATCC highly recommends that protective gloves and clothing always be used and a full face mask always be worn when handling frozen vials. Storage at -70☌ will result in loss of viability. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70☌.
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt.
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